Evolving P450pyr monooxygenase for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol.
نویسندگان
چکیده
Directed evolution of P450pyr created I83M/I82T mutant for highly regioselective terminal hydroxylation of n-butanol to 1,4-butanediol, representing the first achievement of this hydroxylation reaction by chemical or enzymatic methods and an unique example of evolving a hydroxylase to switch the substrate acceptance from a hydrophobic to hydrophilic compound.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 5 شماره
صفحات -
تاریخ انتشار 2015